Analisys of microsatellites markers in INTA Abra Pampa vicuña populations.

LONGO, ANDREA ELENA; BARRERA, ANTONIO DANIEL; VALDECANTOS, PABLO ALBERTO; MICELI, DORA CRISTINA

Tafi del Valle, Tucumán (Argentina)

Jornada; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2006

Asociación de Biología de Tucumán

The breeding of South American Camelids (llama, alpaca, guanaco, vicuna) is a very important economic activity for the socio-economic development of the NOA communities. The vicuna population of INTA Abra Pampa, bred in semi captivity, was originated from a small number of founder animals; being possible to found a low genetic variability. We used microsatellites markers (highly polymorphic nuclear DNA markers found in the chromosomes of most eukaryotes) in order to begin the study of genetic variability in the vicuna population of INTA Abra Pampa. In this work we analyzed two known camelid microsatellite loci (LCA 19 and YWLL 8). Genomic DNA samples were obtained from the fibers roots of 20 vicunas. A fast and simple protocol for DNA extraction was developed using guanidine thiocianate lysis solution and a chloroform purification step. Results showed that the protocol of DNA extraction from the fiber developed in this work is effective and yields good quality DNA. The two microsatellites analyzed showed polymorphism. The numbers of variants corresponding to the loci LCA 19 and YWLL 8 was 9 and 7 respectively. Results obtained as well as standardization of the PCR and electrophoresis conditions will make possible the study of genetic variability in the population of vicunas.