Bifidobacterium CECT 7765 improves metabolic and immunological dysfunction associated with obesity in highfat diet fed mice

GAUFFIN CANO; SANTACRUZ A; TREJO T; SANZ Y

OBESITY

NATURE PUBLISHING GROUP

Año: 2013

1930-7381

The aim of this study was to evaluate the effects of oral administration of Bifidobacteriumpseudocatenulatum CECT 7765 on metabolic and immune dysfunction in mice with highfatdiet (HFD) induced obesity. Adult (age 6?8 week) male wild-type C57BL-6 mice werefed a standard diet (SD) or HFD, supplemented or not with B. pseudocatenulatum CECT7765 for seven weeks. The following parameters were assessed: animal weight, serumbiochemical parameters, liver steatosis, white adipose tissue weight and adipocyte size,lipid micelles per enterocyte, functions of immunocompetent cells, and composition andinflammatory properties of the fecal microbiota. B. pseudocatenulatum CECT 7765administration reduced serum cholesterol, triglyceride and glucose levels by 36, 25 and 35%, respectively in obese mice. This bacterial strain also induced a reduction in insulin andleptin levels in HFD-fed mice. B. pseudocatenulatum CECT7765 significantly reducedliver steatosis, the number of larger adipocytes and number of fat micelles in enterocytes ofobese mice, suggesting reductions in dietary fat absorption. The strain also increased themacrophage oxidative burst, the ability of macrophages and dendritic cells [DCs]) toinduce cytokines (TNF-α) in response to pathogenic bacterial molecules (LPS), and theability of DCs to induce T lymphocyte proliferation. The bacterial strain also increasednumbers of Bifidobacterium spp. and reduced those of Enterobacteriaceae spp. in HFDfedmice, which led to reducing inflammatory signals coming from the gut. In conclusion,B. pseudocatenulatum CECT 7765 was shown to ameliorate both metabolic andimmunological dysfunctions related to obesity in HFD-fed mice.