Macrophages modulate neovascular response in an acute model of imflammation. Expression of pro-angiogenic proteins.

DE LA TORRE EULALIA; HOVSEPIAN EUGENIA; GOREN NORA; SALES MARIA ELENA

Buenos Aires

Congreso; 1er. Congreso Franco-Argentino de Inmunología,; 2010

Sociedad Argentina de Inmunologia - Société Française d?Immunologie.

Septic shock (SS) induced by bacterial infections, affect mainly the cardiovascular system. This damage is caused by microorganisms themselves or their endotoxins, and may be enhanced by the infiltration of immune cells in the myocardium. The role of macrophages (MP) in inflammatory angiogenesis during SS is almost unknown. We investigated the angiogenic abitility of peritoneal MP in a model of acute inflammation induced by lippopolysaccharide (LPS) from E. coli in BALB/c mice. We demonstrated that MP are able to stimulate neovascularization at 5x105 per site, and treatment with LPS (10 ug/ml) in vitro (LPS-MP) reduces MP number required to produce angiogenesis (4x105) potentating this effect (number of skin vessels/mm2) (normal skin: 1.13 ± 0.20; MP: 1.35 ± 0.13; LPS-MP: 2.18 ± 0.19, p