GENISTEIN (GNT) AMELIORATES PARAQUAT (PQ)-INDUCED HEPATIC INJURY IN RATS

SEMENIUK M; CERE, LUCILA INES; CIRIACI N; BUCCI MUÑOZ M; TABORDA D; LUQUITA MG; CATANIA VA; RIGALLI JP; RUIZ ML

Modalidad Virtual

Congreso; Reunion Anual Biociencias 2020; 2020

SAIC-SAI-SAFIS

Previously, we demonstrated that GNT induces expression of hepaticP-glycoprotein (P-gp), a canalicular ABC transporter that extrudestoxicants such as PQ into bile. Aim: To evaluate the hepatoprotectiverole of GNT in a model of acute intoxication with PQ in rats. Methods:Treatment I: Control (vehicles), GNT (5 mg/kg/day sc, 4 days),PQ (50 mg/kg ip, last day) and GNT+PQ. Hepatic malondialdehyde(MDA) levels were measured by thiobarbituric acid reactive substancesmethodology. Levels of 4-hydroxynonenal protein adducts(4-HNEp-add) and glutathione-S-transferase alpha (GSTα) proteinexpression were evaluated in liver by Western blotting. TreatmentII: Control (vehicle), GNT (5 mg/kg/day sc, 3 days). Biliary excretionof PQ was studied in two models: in vivo excretion and isolatedperfused liver (IPL). PQ (50 mg/kg iv) was administered at day 4,before bile sampling. Bile was collected each 10 min, during a 40min-period. PQ was quantified by HPLC. Results (*p