A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus

DALMIRO GOMEZ RIBOT; ESTEBAN DÍAZ, MARÍA VICTORIA FAZIO, CARLOS GRESTA; VALERIA CAREAGA, MARTA MAIER; EVANGELINA CAPOBIANCO, ALICIA JAWERBAUM

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in placental development, but markers of a pro-inflammatory state when produced in excess. Previous studies have established that gestational diabetes mellitus (GDM) induces a pro-inflammatory intrauterine environment. We hypothesized that a diet enriched in olive oil (OO) regulates MMP-2 and MMP-9 activities in placentas and in maternal and cord blood from women with GDM. Fifty control (C) and GDM women were enrolled after signing an informed consent (protocol approved by the Ethics Committee of Hospital Pirovano, Buenos Aires). All of them followed the WHO diet for pregnancy, and a group of GDM women received a 26 mL-OO addition daily from week 24-28 of gestation until delivery (GDMO group). At delivery, placental villous explants and maternal and cord blood were obtained and stored at -80oC for zymography analysis. Fatty acids (FAs) profile was evaluated in cord blood by GC-FID. MMP-9 activity was increased in the placentas and in maternal blood of the GDM group (133 and 81 % respectively, p