Maternal diet enriched in olive oil regulates lipid levels and PPAR target genes expression in fetal livers from gestational diabetic rats.

DAIANA FORNES, SABRINA ROBERTI, ROMINA HIGA, EVANGELINA CAPOBIANCO, ALICIA JAWERBAUM

Congreso; 6th International Symposium on Metabolic Programming and Microbiome. 3rd Meeting of Ibero-American DOHaD chapter; 2018

Gestational diabetes mellitus (GDM) is a prevalent disease that increases the risk of adverse fetal outcomes. We have previously characterized a GDM model obtained through adverse intrauterine programming in the offspring of pregestational diabetic rats. In the fetal liver from GDM rats, we found sex-dependent alterations in the levels of peroxisome proliferator activated receptors (PPARs), master regulators of lipid metabolism. The aim of this work was to evaluate triglycerides and perilipin 2 (PLIN2) levels and the expression of target genes involved in lipid accretion (Fas, Scd-1) and lipid oxidation (Aco, l-Cpt-1) in the fetal liver from GDM rats, as well as the capability of diets enriched in olive oil to prevent possible alterations in these parameters.Pregestational diabetic rats (F0) were obtained through neonatal streptozotocin administration (90mg/kg). The offspring from control (C) and pregestational diabetic rats were mated with healthy males. GDM was induced by intrauterine programming in the offspring (F1) of pregestational diabetic rats. The fetal livers from C and GDM rats were explanted on day 21 of pregnancy. In the liver of male fetuses from GDM rats, we found an increase in the triglycerides and PLIN2 levels (53% and 32%, respectively; p