Peroxynitrites and matrix metalloproteinases in the term placenta from type 2 diabetic patients.

CAPOBIANCO E; SOSA M; BASUALDO N; DI MARCO I; FAINGOLD C

Santiago de Chile

Congreso; International Federation of Placenta Associations (IFPA) Meeting 2010. Fetus and Placenta: A perfect harmony.; 2010

Type 1 pregestational diabetes leads to a pro-inflammatory intrauterine environment, characterized by increased reactive oxygen species (ROS), nitric oxide (NO) overproduction and matrix metalloproteinases (MMP) overactivity. The objective of this work was to address oxidative and nitrative stress in the placenta from Type 2 pregestational diabetes, and whether peroxynitrites regulate the activity of MMP-2 and MMP-9 in the placenta. Methods: Term placental villous tissues from healthy and pregestational type 2 diabetic patients, were isolated and frozen (-80ºC) for further determination of TBARS (an index of lipid peroxidation), nitrates/nitrites (an index of NO production), concentrations of nitrated proteins (enzimoinmunoanalysis and Western Blot). Placental explants were incubated a) in the presence or absence of FeTPPs (a peroxynitrite catalyst) for further determination of nitrates/nitrites and TBARS, and b) in the presence or absence of peroxynitrites (10-100μM) for further determination of MMPs activity by zymography. Results: The placenta from Type 2 diabetic patients showed increased nitrates/nitrites (p