Intergenerational transmission of metabolic alterations in the left ventricle of type 2 diabetic male rats.

IRUNE PIRRONE, CINTIA ROMINA GATTI, MARÍA LAURA LEONARDI, ALICIA JAWERBAUM, EVANGELINA CAPOBIANCO.

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC) en conjunto con las sociedades SAI - FAIC y SAFIS.; 2022

The prevalence of type 2 diabetes (T2D) can be related to the intergenerational transmission of metabolic anomalies that affect the offspring’s health. We previously observed development of T2D in the offspring of female diabetic rats. Diabetic cardiomyopathy (DCM) is described by anomalies in the left ventricle and it is associated with oxidative stress, inflammation, endoplasmic reticulum (ER) stress and other pathologic conditions. Peroxisome proliferator activated receptors (PPARs) are transcription factors related to metabolism and anti-inflammatory processes in the heart. The aim was to evaluate the expression of PPARs, inducible nitric oxide synthase (iNos), the antioxidant enzyme MnSod and genes involved in the ER stress, in the left ventricle of male rats that develop diabetes by intrauterine programming.Methods: The male offspring of female Control (C) and diabetic rats (D: diabetic males obtained by intrauterine programming) were euthanized at 8 months of age (n=8). Triglycerides (TG) and HDL-cholesterol (HDL-c) were measured in plasma by colorimetric assays. The TG/HDL-c ratio was calculated as a measure of insulin resistance. The left ventricle was stored at -80°C for the evaluation of mRNA (by qRT-PCR) of Pparα, Pparγ, Pparδ, iNos, MnSod and ER stress genes (Chop, sXbp1, Atf4). Results: The TG/HDL-c ratio was increased in D males (73% p< 0.05). The expression of Pparα and Pparγ was decreased in the left ventricle of D males (52% and 70%, respectively, p