Modulation of nitric oxide concentration and lipid metabolism by 15deoxy delta12,14PGJ2 in embryos from control and diabetic rats during early organogenesis

JAWERBAUM ALICIA; WHITE VERÓNICA; SINNER DÉBORA; PUSTOVRH CAROLINA; CAPOBIANCO EVANGELINA; GONZÁLEZ ELIDA

REPRODUCTION

SOCIETY FOR REPRODUCTION AND FERTILITY

Lugar: UK; Año: 2002 vol. 124 p. 625 - 631

1470-1626

The concentration of 15-deoxy D12,14PGJ2 (15dPGJ2) andits effects on nitric oxide generation and neutral lipid inembryos from control and neonatal streptozotocin-induced(n-stz) diabetic rats during organogenesis were investigated.15dPGJ2 is produced in embryos during organogenesis,and its production is lower in embryos of n-stzdiabetic rats than in embryos from control rats. Nitrate andnitrite concentrations were higher in embryos from n-stzdiabetic rats and were reduced in the presence of 15dPGJ2both in embryos from control and diabetic rats. Thus,decreased 15dPGJ2 concentrations in embryos from n-stzdiabetic rats may be related to the high nitric oxideconcentrations found in those embryos. Exogenous 15dPGJ2decreased cholesterol and cholesteryl ester concentrationsin embryos from control and n-stz diabetic rats, andreduced triacylglycerol concentrations in control embryos.Incorporation of [14C]acetate into lipids showed decreasedde novosynthesis of cholesteryl ester and triacylglyceridesin embryos from n-stz diabetic rats compared withcontrols. Exogenous 15dPGJ2 reduced the incorporation of[14C]acetate into triacylglycerides, cholesterol and cholesterylester in embryos from both control and n-stz diabeticrats. 15dPGJ2 is present in embryos during organogenesis,and reduces embryonic nitric oxide production and lipidsynthesis. The lower 15dPGJ2 concentration in embryosfrom n-stz diabetic rats may result in developmentalalterations in this diabetic model.