INVESTIGADORES
POLTI Marta Alejandra
artículos
Título:
Intracellular chromium accumulation by Streptomyces sp. MC1
Autor/es:
POLTI, MARTA ALEJANDRA; AMOROSO, MARÍA JULIA; ABATE; CARLOS MAURICIO
Revista:
WATER AIR AND SOIL POLLUTION
Editorial:
Springer Netherlands
Referencias:
Lugar: Dordrecht; Año: 2011 vol. 214 p. 49 - 57
ISSN:
0049-6979
Resumen:
Streptomyces
sp. MC1, previously isolated from sugar cane, has shown ability to
reduce Cr(VI) in liquid minimal medium and soil samples. The objective
of this work was to demonstrate the intracellular chromium accumulation by Streptomyces sp. MC1 under different culture conditions. This strain was able to accumulate up to 3.54
mg of Cr(III) per gram of wet biomass, reducing the 98 % of Cr(VI)
and removing 13.9 % of chromium from the culture medium supernatants. Streptomyces
sp. MC1 chromium bioaccumulation ability was corroborated by using
Timms reagent technique, a low cost method, which has been used by
first time to detect chromium deposits in bacteria. The results of atomic absorption spectrometry, scanning electron microscopy and energy dispersive X-ray analysis suggest that the mechanism of Cr(VI) resistance observed in Streptomyces
sp. MC1 includes adsorption coupled with reduction to Cr(III), and
finally, Cr(III) bioaccumulation. This mechanism have special
relevance to remediation of Cr(VI) contaminated environments by Streptomyces sp. MC1.