Preliminary study of the absolute quantitation of Escharichia coli (EHEC) O157:H7 proteome

CATALDI ANGEL; AMADIO ARIEL FERNANDO; AMIGO NATALIA; JINLONG BEI; ZHANG QI; SILVA M WANDERSON; LARZABAL MARIANO; ZHONGJIAN CHEN

Foz de Iguazu, Paraná, Brasil

Congreso; 29 Congresso Brasileiro de Microbiologia; 2017

Sociedad Brasilera de Microbiologia

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a human pathogen responsible for diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). The infection by EHEC is widely distributed in the world and numerous outbreaks of enterohemorrhagic disease were reported. Argentina is considered the country with the highest HUS incidence in the world [1]. Proteomic studies show that absolute quantitation of proteins present within a complex protein mixture is extremely important for understating physiological adaptations in response to biological demands promoted by environmental changes. Thus in this study, to promote the more comprehensive insight into physiological and pathogenesis molecular basis of EHEC O157:H7, we applied high-throughput proteomics, using iTRAQ-based quantitative proteomic analysis to estimate the absolute abundance of EHEC O157:H7 proteome.From our proteomic analysis, we identified 2,234 non-redundant protein of EHEC O157:H7, with had at least two unique peptide. When compared this result with in silico data of EHEC O157:H7 genome was characterized approximately 40% of the predicted proteome of this pathogen.The result obtained to date show important proteins that contribute to physiology and pathogenicity of EHEC O157:H7.