Carbachol stimulates inositol phosphate formation transiently in Trypanosoma cruzi.

MONICA GARRIDO, MARIANA BOLLO, AND ESTELA E. MACHADO-DOMENECH.

CELLULAR AND MOLECULAR BIOLOGY

C.M.B. Association

Lugar: Poitiers; Año: 1996 vol. 42 p. 221 - 225

0145-5680

Carbachol stimulates inositol phosphate formation transiently in Trypanosoma cruzi Mónica Garrido, Mariana I., Bollo, and Estela E. Machado-Domenech. Cell. Mol. Biol. 42: 221-225. (1996). The effect on carbachol on Trypanosoma cruzi epimastigote forms was studied. Suspension of epimastigote cells prelabelled with myo[3H]-inositol were incubated at 28°C in the presence of carbachol. The levels of inositol phosphate after the challenge of carbachol were determinated to see if the accumulation of inositol phosphate was time-dependent. In the presence of Li+ the levels of IP3 and IP4 were significantly increased during the 3 min with a peak at 1 min after the charbacol challenge. This effect was suppressed by atropine. The results show that charbacol influence rapid changes in the phosphoinositide metabolism and they are time dependent involving second messenger systems associated with the generation of IP3. In addition they would suggest that this IP3 generation is through a receptor-mediated process linked to phospholipase C by a G-protein. Key words: Trypanosoma cruzi, inositol phosphates,carbachol, phospholipase C.