Biphasic and dose-dependent accumulation of InsP3 in Trypanosoma cruzi stimulated by a synthetic peptide carrying a chicken aD-globin fragment.

MARIANA GARRIDO, MARIANA I.BOLLO,AND ESTELA E. MACHADO-DOMENECH.

CELLULAR AND MOLECULAR BIOLOGY

C.M.B association

Lugar: Poitiers; Año: 1996 vol. 42 p. 859 - 864

0145-5680

Biphasic and dose-dependent accumulation of InsP3 in Trypanosoma cruzi stimulated by a synthetic peptide carrying a chicken aD-globin fragment.   Mónica M. Garrido, Mariana I Bollo, and Estela E. Machado-Domenech (1996). Cell. Mol. Biol. 42: 859-864.   Abstract – A synthetic peptide corresponding to residues 1 to 40 of chicken aD-globin stimulated the accumulation of inositol phosphate (InsPs) in Trypanosoma cruzi epimastigotes. This accumulation was ahown to be biphasic and dose-dependent. The enhanced inositol trisphosphate (InsP3) accumulation occurred at the third minute with a subsequent increase which is not transient, but sustained over a much longer period. Another peptide carrying residues 35-73 was less active. These results evidenced the activation of the phosphoinositide phospholipase C acivity in response to peptides that bind specifically to T.cruzi epimastigote cells.   Key word: Trypanosoma cruzi, inositol phosphates, phospholipase C, differentiation