Cytotoxic activity of the organic extract and isolation of an active flavonoid from Mikania periplocifolia (Asteraceae)

LAURELLA CECILIA; MARTINO RENZO; BEER F.; SELENER, M; ALONSO MARÍA ROSARIO; CATALAN, CESAR; MARTINO, VIRGINIA; ANESINI CLAUDIA; SULSEN, VALERIA

Buenos Aires

Conferencia; VI International Conference on Polyphenols anda Health ICPH-6; 2013

Facultad de Farmacia y Bioquímica UBA e Instituto de Bioquímica y Medicina Molecular UBA - Conicet

Cytotoxic activity of the organic extract and isolation of an active flavonoid from Mikania periplocifolia (Asteraceae) Laurella L.1, Martino R.2, Beer F.1, Selener M.1, Alonso R.2, Catalan C.3, Martino V.1,2, Anesini C.2, Sülsen V.1,2. 1. Cátedra de Farmacognosia, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 2°P (1113) Buenos Aires, Argentina. 2. Instituto de Química y Metabolismo del Fármaco (IQUIMEFA) (CONICET-UBA), Junín 956 2°P (1113) Buenos Aires, Argentina. 3. INQUINOA-CONICET, Instituto de Química Orgánica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 471 (T4000INI), San Miguel de Tucumán, Argentina. According to the World Health Organization, lymphoma stands at the fifth place of cancer type related to death over the world. It is necessary to search new and more selective drugs with fewer adverse effects to treat this disease. Natural products play an important role in the process of discovering and developing new drugs. In this sense, the aim of the present study was to isolate cytotoxic compounds from Mikania periplocifolia. The aerial parts of M. periplocifolia were extracted with dichloromethane and this extract was fractionated by column chromatography (CC). After purification process, a yellow powder (compound A) was obtained. Cytotoxic activity of the extract and the isolated compound was investigated in a murine lymphocitic leukaemia cell line (EL-4) by MTT assay. The organic extract of M. periplocifolia inhibited by more than 75% cell proliferation at a concentration of 100 µg/ml. The chromatographic behaviour UV analysis of compound A allowed us to identify it as a methoxy tetrahydroxylated flavone. This compound showed a 50% effective concentration (EC50) of 20.02 µg/ml when tested for its in vitro cytotoxic activity. This is the first time that the cytotoxic activity and the isolation of a bioactive flavone from M. periplocifolia are reported. Acknowledgments. This work was supported by PICT 2011-1753 (Agencia Nacional de Promoción Científica y Tecnológica) and UBACYT 20020110200114.