PROTECTIVE ACTIVITY ELICITED BY CANNABIS SATIVA AND TILIA X VIRIDIS EXTRACTS AGAINST GLUTAMATE INDUCED OXIDATIVE STRESS IN HT-22 NEURONS

SAINT MARTIN, ELINA MALÉN; BARREIRO ARCOS L; PERALTA IGNACIO; MARRASINI CARLA; COGOI LAURA; ALONSO MARÍA ROSARIO; ANESINI CLAUDIA

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

SAIC SAB AAFE AACYTAL

Oxidative stress (OS) affects the central nervous system in epilepsy. The prevention of neuronal cell death induced by OS might be an interesting therapeutic approach in the treatment of this and other neural disorders. Cannabis sativa L. is used in the treatment of epilepsy, being cannabidiol (CBD) its main anticonvulsant compound. Tilia x viridis is widely distributed in Argentina and has antioxidant and sedative activities. The aim of this work was to evaluate the effect of an ethanolic extract of C. sativa (CSRD), and an aqueous extract of T. x viridis (TE) and their association on the OS induced by glutamate (Glu) in the HT-22 cell line.The main compounds in CSRD and TE were identified and quantified by HPLC-MS/MS and HPLC-UV. Cells were pre-incubated with the extracts for 2 hs and challenged with Glu 5 mM for 12 or 24 hs to assess: cell viability with MTT spectroscopically, and reactive oxygen species (ROS) with 2´,7´-dichlorodihydrofluorescein diacetate (DCF-DA) in a fluorescence reader and microscope. Results were expressed as media ± SEM. *p< 0,05, ****p