Antioxidant Effect of Cannabis sativa L. Resin Associated with Larrea divaricata Cav. Extract: Synergism, Additivity and Antagonism

MARTIN, MALÉN SAINT; MARRASSINI, CARLA; SOFRÁS, FRESIA M. SILVA; PERALTA, IGNACIO; COGOI, LAURA; BAREN, CATALINA VAN; ALONSO, MARÍA R.; ANESINI, CLAUDIA

International Journal of Pharmaceutical Sciences Review and Research

IJPSRR

Lugar: Hyderabad; Año: 2022 vol. 77 p. 1 - 10

Cannabis sativa L. (Cannabaceae) is used as anticonvulsant in refractory epilepsy. Convulsions induce oxidative stress in the brain. Synergistic interactions between plants could improve the therapeutic and reduce adverse effects. Larrea divaricata Cav.’s (Zygophylliaceae) antioxidant activity is well-documented. The aim of this work was to evaluate the antioxidant activity of an ethanolic extract of C. sativa (CSR), study cannabidiol’s (CBD) participation and evaluate the possible synergistic effect of CSR with an aqueous extract of L. divaricata (LE). Antioxidant activity was determined by the 1,1-diphenyl-2-picryhydrazyl (DPPH) and the lipid peroxidation assays. For the DPPH assay, the inhibitory concentration 50 (IC50) were: CSR=19.82 ±1.47 µg/ml, 24.18±0.54µg/ml, CBD=12.49 ± 0.79µg/ml. The IC50 values for the combinations: CSR + LE 25 µg/ml=2.91±0.10µg/ml (p