Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus lineatus (Pisces, Curimatidae).

M. F. SIMONIELLO ; GIGENA, F.; POLENTA, G..; LOTESTE A.; KLEINSORGE E. ;CAMPANA, M.; SCAGNETTI J. AND PARMA, M. J.

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

Springer

Lugar: USA; Año: 2009 vol. 83 p. 155 - 158

0007-4861

 Toxicants on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using alkaline comet assay applied on erythrocytes after in vivo exposure of Prochilodus lineatusto different concentrations of Cypermethrin (0.300, 0.150, 0.075 and 0.000 lg/L) as a probable chemical mutagen.The results revealed a significantly higher level of DNA damage at all concentrations of Cypermethrin tested compared to control and background level (p\0.05). We have standardized the technique for one of the most common native fish species that will be useful for biomonitoringgenotoxicity in polluted waters of the region