Comparative Enzymatic and Toxic Activities of Bothrops Venoms from Argentina

DE ROODT, ADOLFO; DOLAB, JORGE; SEGRE, LILIANA; LITWIN, S.; ACCATTOLI, C.; DOKMETJIAN, CRISTIAN; ZUÑIGA,S.; GOULD, EDUARDO; GOULD, JORGE; DINAPOLI, HERNÁN; HEKER, J.; VIDAL, JUAN CARLOS

Praga

Congreso; Third World Congress of Herpetology; 1997

Some toxic and enzymatic activities were determined with the crude venoms of Bothrops altematus (AL); B. ammodytoides (AM); B. jararaca (JA); B. jararacussu (JC); B. moojeni (M) and B. neuwiedii (N). The toxic activities measured were: LD 50 (i.v., in CF-1 micc ): Minimal Hemorragíc Dose (MHD in CF-1 mice); Minimal Necrotizing Dose (MND, in CF-1 mice) and Minimal procoagulant dose in human plasma (MPD-P). The enzymatic activities measured were: Proteolytic (P, on gelatin), Phospholipase (PLA on egg yo1k), Indirect Hemolysis (1 H, on human erythrocytes plus egg-yolk) and Radial Hemolysis (R.H. on human erythrocytes plus egg yolk in 1 % Agarose). LD50 (glg) 0.5 (AM); 1.13 (AL): 1.42 (JA); 0.35 (JC); 1.5 (M);1.25 (N). MND( tg) : 73 (AL); 38 (AM): 73 (JA); 19 (JC): 55 (M): 37 (N).MHD ütg): 14 (AL); 10 (AM); 17(JA); 32 (JC); 34 (M); 9 (N). MPD-P (mg/1) 27.4 (AL); 30 (AM): 59.8 (JA); 45 (JC); 72.6 (M); 37.4 (N). Pg(/2.0 m1 gclatin): 12.5 (AL); 12.5 (AM); 400 (JA); 600 (JC): 400 (M); 25 (N); PLA (units/ml): 5.1 (AL);110.3 (AM); 5.2 (J A); 35.4 (JC):65.9 (M); 110.2 (N). R. H.(G): 70 (AL); 18.8 (AM): 280 (JA); 130 (JC); 70 (M); 25 (N). Ul.( g) 100 (AL); 50 (AM); l00 (JA); 50 (JC); 100 (M): 25 (N). Rc1ationships between 1ethality a nd the different enzymatic and toxic activities are discussed.