ALOYSIA POLYSTACHYA ANTI-CANCER ACTION IN COLORECTAL CANCER

ALEJANDRA GRACIELA PALMA; JULIANA BERNACCHIA; MILENI SOARES MACHADO; PAOLA DI LEO LIRA;; MOSCATELLI VALERIA; MARÍA CECILIA LIRA; FRANCISCO DAMIÁN ROSA; IARA CASTELLANOS; MARÍA FLORENCIA QUINTANILLA; MARÍA FERNANDA RUBIO; DAIANA RETTA; MÓNICA ALEJANDRA COSTAS

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2022 vol. 82

0025-7680

Previously we have shown that extracts from the plant Aloysia polystachya (AP) have a cytotoxic action in colorectal cancer cellsbut its lower in non-tumoral cells. The coactivator of nuclear receptors RAC3 is overexpressed in several types of cancer, like colorectal cancer, but its expression is low in non-tumoral cells. Thismolecule is co-activator of the Aryl Hydrocarbon Receptor (AHR),a modulator of intestinal balance, whose expression is increased intumors. The aim of this work was to investigate the molecular mechanisms of AP cytotoxic action and its possible components involvedwith this effect in colorectal cancer. Since RAC3 expression and APcytotoxicity are low in non-tumoral cells, and RAC3 is coactivator ofAHR and NF-ĸB, we first decided to analyze if these molecules wereinvolved in AP effect. When colorectal cancer HCT116 cells wereincubated with AP extract we found by immunofluorescence assaythat the nuclear translocation of these molecules were increasedand that AHR and RAC3 co-localized in these cells. Then, we compared the biological activity of two AP plants grown in different partsof Argentina and found that both were capable to induce cytotoxicity in HCT116 cells (1:2 dilution % cytotoxicity: Buenos Aires 46%,p