PERSONAL DE APOYO
GRILLO Claudia Alejandra
artículos
Título:
Does over-exposure to copper ions released from metallic copper induce cytotoxic and genotoxic effects on mammalian cells?
Autor/es:
C.A. GRILLO, M.A. REIGOSA AND M. FERNÁNDEZ LORENZO DE MELE
Revista:
CONTRACEPTION
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2010 vol. 81 p. 343 - 349
ISSN:
0010-7824
Resumen:
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Background: A high dissolution of copper from intrauterine devices (IUDs)
occurs during the first days after insertion. This work is focused on the
assessment of the possible cyto- and genotoxic effects of different
concentrations of copper ions released from metallic copper on mammalian cells
in vitro.
Study desing: MTT / Trypan blue tests and Comet assay were used to evaluate
potential cytotoxicity and genotoxicity respectively in Chinese hamster ovary
cells (CHO-K1).
Results: Reduction of mitochondrial activity by copper ions was observed for
extracts at ³7.42 mg/L concentrations, while Trypan
blue exclusion test for plasma membrane integrity showed significant decrease
in cell viability (c.a. 90%) for 10.85 mg/L concentration. Additionally,
copper-induced DNA damage was detected for 5.67-7.42 mg/L concentration range.
Conclusion: Our results demonstrate cytotoxic and genotoxic effects of copper
ions released from metallic copper on CHO-K1 cells and emphasize the importance
of reducing the initial copper dissolution from IUD without affecting the
contraceptive action.
