PERSONAL DE APOYO
GRILLO Claudia Alejandra
artículos
Título:
Genotoxic Effects of Benzo[a]pyrene and Dibenzo[a,l]pyrene in a Human Lung Cell Line
Autor/es:
S. A. MOURÓN, C. A. GRILLO, F.N. DULOUT, AND C. D. GOLIJOW
Revista:
INTERNATIONAL JOURNAL OF TOXICOLOGY
Editorial:
SAGE PUBLICATIONS INC
Referencias:
Año: 2006 vol. 25 p. 49 - 55
ISSN:
1091-5818
Resumen:
<!--
/* Font Definitions */
@font-face
{font-family:Times-Roman;
panose-1:0 0 0 0 0 0 0 0 0 0;
mso-font-charset:0;
mso-generic-font-family:roman;
mso-font-format:other;
mso-font-pitch:auto;
mso-font-signature:3 0 0 0 1 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-parent:"";
margin:0cm;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";
mso-ansi-language:EN-GB;}
p.MsoBodyText, li.MsoBodyText, div.MsoBodyText
{margin:0cm;
margin-bottom:.0001pt;
text-align:justify;
line-height:150%;
mso-pagination:widow-orphan;
mso-layout-grid-align:none;
text-autospace:none;
font-size:12.0pt;
mso-bidi-font-size:9.0pt;
font-family:Times-Roman;
mso-fareast-font-family:"Times New Roman";
mso-bidi-font-family:"Times New Roman";
mso-ansi-language:EN-US;}
@page Section1
{size:612.0pt 792.0pt;
margin:70.85pt 3.0cm 70.85pt 3.0cm;
mso-header-margin:35.4pt;
mso-footer-margin:35.4pt;
mso-paper-source:0;}
div.Section1
{page:Section1;}
-->
Several studies have shown that polycyclic aromatic
hydrocarbons (PAHs) produce genotoxic effects in assays performed in vivo and
in vitro. This study was undertaken to investigate the ability of benzo[a]pyrene
(BP) and dibenzo[a,l]pyrene (DBP) to induce DNA damage in a human lung
fibroblast cell line (MRC-5), using sister-chromatid exchanges test (SCEs), the
comet assay, and evaluating point mutations in codon 12 of the K-ras protooncogene
by polymerase chain reactionsingle-strand conformation polymorphisms (PCR-SSCPs)
and restriction fragment length polymorphisms (RFLP)-enriched PCR methods. Sister-chromatid
exchanges frequencies were significantly increased in cells exposed to benzo[a]pyrene
and dibenzo[a,l]pyrene in relation to controls (p <.001).
Using the standard alkaline comet assay, significant differences between groups
were found for the variable comet moment (CM)when cells were exposed toBP(p <.001)
andDBP(p <.001). Nevertheless, PCR-SSCP and RFLP-enriched PCR methods
did not show any association between treatments with BP and DBP and K-ras point
mutations. The data presented in this study indicated that BP and DBP induced
both DNA strand breaks and sister-chromatid exchanges but not significant point
mutations at codon 12 of K-ras gene in the MRC-5 cell line.
