Nephrotic síndrome induced by doxorubicin in rats. Preliminary study of the effects on proximal tubules

COSTALES, C; ZOTTA, E; DIAZ, A; SEYAHIAN, A; OCHOA, F; IARIA, A; LAGO, N

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Introduction. Nephrotic syndrome is a consequence of renal dam-􀁄􀁊􀁈􀀏􀀃 􀁚􀁌􀁗􀁋􀀃 􀁓􀁕􀁒􀁗􀁈􀁌􀁑􀁘􀁕􀁌􀁄􀀃 􀀡􀀃 􀁒􀁉􀀃 􀀖􀀃 􀁊􀀒􀁇􀀏􀀃 􀁄􀀃 􀁕􀁈􀁇􀁘􀁆􀁈􀁇􀀃 􀁆􀁒􀁑􀁆􀁈􀁑􀁗􀁕􀁄􀁗􀁌􀁒􀁑􀀃 􀁒􀁉􀀃 􀁅􀁏􀁒􀁒􀁇􀀃􀁓􀁕􀁒􀁗􀁈􀁌􀁑􀁖􀀏􀀃 􀁋􀁌􀁊􀁋􀀃 􀁏􀁈􀁙􀁈􀁏􀁖􀀃 􀁒􀁉􀀃 􀁆􀁋􀁒􀁏􀁈􀁖􀁗􀁈􀁕􀁒􀁏􀀃 􀁄􀁑􀁇􀀃 􀁗􀁕􀁌􀁊􀁏􀁜􀁆􀁈􀁕􀁌􀁇􀁈􀁖􀀏􀀃 􀁚􀁌􀁗􀁋􀀃 􀁄􀀃 􀁋􀁌􀁊􀁋􀁈􀁕􀀃risk of blood clotting.Doxorubicin is an anthracycline used for thetreatment of solid and hematological tumors. The mechanisms of 􀁄􀁆􀁗􀁌􀁒􀁑􀀃􀁓􀁕􀁒􀁓􀁒􀁖􀁈􀁇􀀃􀁉􀁒􀁕􀀃􀁗􀁋􀁈􀀃􀁄􀁑􀁗􀁋􀁕􀁄􀁆􀁜􀁆􀁏􀁌􀁑􀁈􀁖􀀃􀁄􀁕􀁈􀀃􀁗􀁋􀁈􀀃􀁌􀁑􀁗􀁈􀁕􀁓􀁒􀁖􀁌􀁗􀁌􀁒􀁑􀀃􀁅􀁈􀁗􀁚􀁈􀁈􀁑􀀃􀁅􀁄􀁖􀁈􀀃􀁓􀁄􀁌􀁕􀁖􀀃􀁒􀁉􀀃􀁑􀁘􀁆􀁏􀁈􀁌􀁆􀀃􀁄􀁆􀁌􀁇􀁖􀀃􀁚􀁌􀁗􀁋􀀃􀁌􀁑􀁋􀁌􀁅􀁌􀁗􀁌􀁒􀁑􀀃􀁒􀁉􀀃􀀧􀀱􀀤􀀃􀁄􀁑􀁇􀀃􀀵􀀱􀀤􀀃􀁖􀁜􀁑􀁗􀁋􀁈-sis, DNA alkylation, cellular apoptosis and free radicals synthesis. At􀁗􀁋􀁌􀁖􀀃 􀁐􀁒􀁐􀁈􀁑􀁗􀀏􀀃 􀁌􀁗􀀃 􀁌􀁖􀀃 􀁑􀁒􀁗􀀃 􀁎􀁑􀁒􀁚􀁑􀀃 􀁗􀁋􀁈􀀃 􀁄􀁆􀁘􀁗􀁈􀀃 􀁓􀁋􀁜􀁖􀁌􀁒􀁓􀁄􀁗􀁋􀁒􀁏􀁒􀁊􀁌􀁆􀁄􀁏􀀃 􀁐􀁈􀁆􀁋􀁄-nisms involved in tubular damage caused by doxorubicin.􀀷􀁋􀁈􀀃􀁄􀁌􀁐􀀃􀁒􀁉􀀃􀁒􀁘􀁕􀀃􀁚􀁒􀁕􀁎􀀃􀁚􀁄􀁖􀀃􀁗􀁒􀀃􀁖􀁗􀁘􀁇􀁜􀀃􀁗􀁋􀁈􀀃􀁕􀁈􀁖􀁓􀁒􀁑􀁖􀁈􀀃􀁒􀁉􀀃􀁓􀁕􀁒􀁛􀁌􀁐􀁄􀁏􀀃􀁗􀁘􀁅􀁘􀁏􀁈􀁖􀀃under the effects of doxorubicin in rats.Materials and methods. We􀁘􀁖􀁈􀁇􀀃 􀀔􀀓􀀃 􀀶􀁓􀁕􀁄􀁊􀁘􀁈􀀃 􀀧􀁄􀁚􀁏􀁈􀁜􀀃 􀁐􀁄􀁏􀁈􀀃 􀁕􀁄􀁗􀁖􀀃 􀀋􀀕􀀖􀀓􀀐􀀕􀀗􀀓􀁊􀀌􀀃 􀁇􀁌􀁙􀁌􀁇􀁈􀁇􀀃 􀁌􀁑􀁗􀁒􀀃 􀁗􀁚􀁒􀀃􀁊􀁕􀁒􀁘􀁓􀁖􀀑􀀃􀀨􀁛􀁓􀁈􀁕􀁌􀁐􀁈􀁑􀁗􀁄􀁏􀀃􀁊􀁕􀁒􀁘􀁓􀀃􀁚􀁄􀁖􀀃􀁈􀁑􀁇􀁒􀁙􀁈􀁑􀁒􀁘􀁖􀁏􀁜􀀃􀁌􀁑􀁒􀁆􀁘􀁏􀁄􀁗􀁈􀁇􀀃􀁚􀁌􀁗􀁋􀀃􀀚􀀑􀀘􀀃􀁐􀁊􀀒􀀮􀁊􀀃 􀁒􀁉􀀃 􀁇􀁒􀁛􀁒􀁕􀁘􀁅􀁌􀁆􀁌􀁑􀀑􀀃 􀀦􀁒􀁑􀁗􀁕􀁒􀁏􀀃 􀁊􀁕􀁒􀁘􀁓􀀃 􀁚􀁄􀁖􀀃 􀁌􀁑􀁒􀁆􀁘􀁏􀁄􀁗􀁈􀁇􀀃 􀁚􀁌􀁗􀁋􀀃 􀁖􀁄􀁏􀁌􀁑􀁈􀀃􀁖􀁒􀁏􀁘􀁗􀁌􀁒􀁑􀀑􀀃 􀀷􀁋􀁈􀀃 􀁄􀁑􀁌􀁐􀁄􀁏􀁖􀀃 􀁚􀁈􀁕􀁈􀀃 􀁐􀁄􀁌􀁑􀁗􀁄􀁌􀁑􀁈􀁇􀀃 􀁌􀁑􀀃 􀁆􀁒􀁑􀁗􀁕􀁒􀁏􀁏􀁈􀁇􀀃 􀁆􀁒􀁑􀁇􀁌􀁗􀁌􀁒􀁑􀁖􀀃 􀁒􀁉􀀃􀁏􀁌􀁊􀁋􀁗􀀏􀀃 􀁗􀁈􀁐􀁓􀁈􀁕􀁄􀁗􀁘􀁕􀁈􀀏􀀃 􀁉􀁕􀁈􀁈􀀃 􀁄􀁆􀁆􀁈􀁖􀁖􀀃 􀁗􀁒􀀃 􀁚􀁄􀁗􀁈􀁕􀀃 􀁄􀁑􀁇􀀃 􀁉􀁒􀁒􀁇􀀃 􀁉􀁒􀁕􀀃 􀀔􀀃 􀁚􀁈􀁈􀁎􀀑􀀃 􀀕􀀗􀀃􀁋􀁒􀁘􀁕􀁖􀀃􀁓􀁕􀁌􀁒􀁕􀀃􀁗􀁒􀀃􀁖􀁄􀁆􀁕􀁌􀃀􀁆􀁈􀀏􀀃􀁕􀁄􀁗􀁖􀀃􀁚􀁈􀁕􀁈􀀃􀁎􀁈􀁓􀁗􀀃􀁌􀁑􀀃􀁐􀁈􀁗􀁄􀁅􀁒􀁏􀁌􀁆􀀃􀁆􀁄􀁊􀁈􀁖􀀃􀁗􀁒􀀃􀁒􀁅􀁗􀁄􀁌􀁑􀀃urine samples to determine proteinuria. Histological and immuno-􀁋􀁌􀁖􀁗􀁒􀁆􀁋􀁈􀁐􀁌􀁆􀁄􀁏􀀃􀁖􀁗􀁘􀁇􀁌􀁈􀁖􀀃􀁚􀁈􀁕􀁈􀀃􀁓􀁈􀁕􀁉􀁒􀁕􀁐􀁈􀁇􀀃􀁗􀁒􀀃􀁇􀁈􀁗􀁈􀁆􀁗􀀃􀁐􀁈􀁊􀁄􀁏􀁌􀁑􀀃􀁄􀁑􀁇􀀃􀀩􀁆􀀵􀁑􀀃􀁕􀁈􀁆􀁈􀁓􀁗􀁒􀁕􀁖􀀃􀁌􀁑􀀃􀁓􀁕􀁒􀁛􀁌􀁐􀁄􀁏􀀃􀁗􀁘􀁅􀁘􀁏􀁈􀁖􀀑􀀵􀁈􀁖􀁘􀁏􀁗􀁖􀀑􀀃􀀵􀁈􀁖􀁘􀁏􀁗􀁖􀀃􀁖􀁋􀁒􀁚􀁈􀁇􀀃􀁄􀁑􀀃􀁌􀁑􀁆􀁕􀁈􀁄􀁖􀁈􀀃􀁌􀁑􀀃 􀁓􀁕􀁒􀁗􀁈􀁌􀁑􀁘􀁕􀁌􀁄􀀃 􀁌􀁑􀀃 􀁈􀁛􀁓􀁈􀁕􀁌􀁐􀁈􀁑􀁗􀁄􀁏􀀃 􀁕􀁄􀁗􀁖􀀃 􀀋􀁓􀀟􀀓􀀑􀀓􀀘􀀌􀀃 􀁚􀁌􀁗􀁋􀀃 􀁑􀁒􀀃 􀁇􀁌􀁉􀁉􀁈􀁕􀁈􀁑􀁆􀁈􀁖􀀃 􀁌􀁑􀀃the excreted volume. We detected proximal tubules cytoplasmatic􀁊􀁕􀁄􀁑􀁘􀁏􀁖􀀏􀀃􀁆􀁜􀁖􀁗􀁌􀁆􀀃􀁇􀁌􀁏􀁄􀁗􀁌􀁒􀁑􀀃􀁚􀁌􀁗􀁋􀀃􀁌􀁑􀁗􀁕􀁄􀁏􀁘􀁐􀁌􀁑􀁄􀁏􀀃􀁓􀁕􀁒􀁗􀁈􀁌􀁑􀁖􀀑􀀃􀀪􀁏􀁒􀁐􀁈􀁕􀁘􀁏􀁌􀀃􀁖􀁋􀁒􀁚􀁈􀁇􀀃mesangial proliferation and segmental necrosis. The immunohistochemistryexpression of megalin and FcRn endocitotic receptors in􀁓􀁕􀁒􀁛􀁌􀁐􀁄􀁏􀀃􀁗􀁘􀁅􀁘􀁏􀁈􀁖􀀃􀁚􀁈􀁕􀁈􀀃􀁌􀁑􀁆􀁕􀁈􀁄􀁖􀁈􀁇􀀃􀀋􀁓􀀟􀀃􀀓􀀑􀀓􀀔􀀌􀀑􀀦􀁒􀁑􀁆􀁏􀁘􀁖􀁌􀁒􀁑􀀑􀀃􀀷􀁋􀁈􀀃􀁇􀁈􀁙􀁈􀁏􀁒􀁓􀀃􀁒􀁉􀀃􀁖􀁌􀁊􀁑􀁌􀃀􀁆􀁄􀁑􀁗􀀃􀁓􀁕􀁒􀁗􀁈􀁌􀁑􀁘􀁕􀁌􀁄􀀃􀁄􀁑􀁇􀀃􀁗􀁋􀁈􀀃􀁌􀁑􀁆􀁕􀁈􀁄􀁖􀁈􀀃􀁌􀁑􀀃􀁗􀁋􀁈􀀃􀁈􀁛􀁓􀁕􀁈􀁖􀁖􀁌􀁒􀁑􀀃􀁐􀁈􀁊􀁄􀁏􀁌􀁑􀀃and FcRn, could be an adaptive mechanism involved in the earlystages of nephrotic syndrome induced by doxorrubicin. More studiesare necessary to clarify the doxorrubicin effects.