Proteolipid Protein as a marker of olfactory bulb granule cell progenitors during adult neurogenesis

LUCILA BROCARDO; CHIARA M PESSANO; LORENA RELA

Córdoba

Congreso; XXXIII Congreso Anual; 2018

Sociedad Argentina de Investigación en Neurociencias

The subventricular zone is a neurogenic niche that produces olfactory bulb interneurons throughout life. Stem cells that express glial fibrilary acidic protein (GFAP) generate transit amplifying progenitors that divide to produce neuroblasts, which in turn migrate to the olfactory bulb via the rostral migratory stream, mature, and integrate to the local circuit as granule and periglomerular neurons. Outside this neurogenic niche, neurogenesis of pyriform cortex pyramidal neurons has been shown to occur in the adult stage and involve progenitors expressing the oligodendrocyte marker proteolipid protein (PLP). Here we ask whether PLP-expressing progenitors can generate new olfactory bulb interneurons. We used a tamoxifen-inducible PLP-Cre mouse line crossed with a Cre-reporter line (LSL-tdTomato) to label PLP-expressing cells and looked for labeled olfactory bulb interneurons at an early (1 week) and late (1 month) time points after induction. We found tdTomato-labeled spiny cells with a morphology compatible with olfactory bulb granule cells at the late but not at the early time point. We will: 1. Test whether tdTomato-labeled cells at the late time point express characteristic markers of olfactory granule cells and 2. Address whether tdTomato-labeled cells at the early time point are found in the subventricular zone and/or rostral migratory stream co-expressing markers characteristic of transit amplifying progenitors and/or neuroblasts of the olfactory bulb neuronal lineage.