Effect of nitrosative stress on AQP9 expression in human trophoblast

MASKIN B; DI PAOLA M; MEDINA Y; CASTRO PARODI M, ABAD C, DAMIANO A

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017

SAIC

Preeclamptic placentas are characterized by an abnormal trophoblastic invasion of the spiral arteries resulting in an inadequate utero-placental blood flow. Consequently, an intermittent perfusion is established which leads to an ischemia/reperfusion injury in the syncytiotrophoblast. Fluctuations in O2 tensions may increase the generation of reactive nitrogen species (RNS) by promoting the formation of peroxynitrite (ONOO-) which could damage lipids, proteins and DNA. Recently, we reported that placental explants exposed to hypoxia/reoxygenation showed an overexpression of AQP9 with a lack of functionality similar to that observed in preeclamptic placentas. However, the mechanisms by which it occurs are still unknown.Our aim was to evaluate the effect of nitrosative stressmediated by ONOO- on AQP9 expression.This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. A. Posadas. Placental explants were cultured in complete DMEM-F12. MgSO4 was added to neutralize the effect of ONOO-, since it is used extensively for prevention and treatment of eclampsia, associated with a reduction in maternal and fetal morbidity. Lipid peroxidation (TBARS) was determined. Expressions of superoxide dismutase 1 (SOD-1), as a measure of the antioxidant enzyme defense system, and AQP9 were also studied.In the presence of 100 μM of ONOO-, AQP9 expression significantly increased 75.42 ± 0.75% compared to control (n=7; p