ROLE OF THE CAVEOLAE IN THE REMODELING OF MATERNAL SPIRAL ARTERIES DURING THE FORMATION OF HUMAN PLACENTA

ALICIA E. DAMIANO; ALEJANDRA RECA; NORA MARTINEZ; NATALIA BELTRAMONE; JULIETA REPPETTI; NORA MARTINEZ; NATALIA BELTRAMONE; JULIETA REPPETTI; FERNANDA PARBORELL; ABRIL SEYAHIAN; FERNANDA PARBORELL; ABRIL SEYAHIAN; ALICIA E. DAMIANO; ALEJANDRA RECA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAFIS

Extravillous trophoblast (EVTs) cells of the human placenta migrateand invade the uterine decidua and adopt an endothelial phenotypeto modify utero-placental arteries. These events also require asuccessful cooperation between EVTs and endothelial cells (ECs).Caveolae are membrane domains that compartmentalize intracellularsignaling pathways to orchestrate different cellular events, ascell migration and invasion. However, the importance of caveolaeduring placentation remains unknown.Our aim was to evaluate the role of caveolae in EVTs migration,endovascular differentiation and interaction with ECs.Swan 71 cell line was treated with 5 mM methyl-beta-cyclodextrin(MCD) to disrupt caveolae. Conditioned medium (CM) was collected.Cav-1 expression was tested by Western blot and immunofluorescence.Migration was assessed by wound healing assay.Endovascular differentiation was evaluated by formation of tube-likestructures in plates coated with Matrigel.To evaluate the interaction between EVTs and ECs (EA.hy926),proliferation (evaluated by MTT assay), migration and tube formationassays were performed in the presence of CM obtained fromSwan 71 cells.Disruption of caveolae significantly decreased Cav-1 expression(n=5; p