Study of cortical oscillatory activity in the development of dyskinesias associated to L-Dopa treatment in a rodent model of Parkinson's disease

BELLUSCIO MA; PIÑA-NOVO D; MURER MG

buenos aires

Congreso; XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; 2017

Sociedad Argentina de Investigación en Neurociencia

Prolonged treatment with L-Dopa in Parkinson´s disease (PD) often leads to the emergence ofabnormal involuntary movements known as L-Dopa-induced dyskinesias (LIDs). Recentfindings in a rodent model of PD have shown that dyskinetic symptoms are linked to theoccurrence of a prominent 80Hz oscillatory local field potential (LFP) activity within theprimary motor cortex. Additionally, L-Dopa treatment decreases exaggerated beta (13-30Hz)oscillatory activity, which is exacerbated in parkinsonian state and has been associated withrigidity and bradykinesia. Despite of this, the correlation between motor symptoms andoscillatory activity in the development of LIDs is not fully understood; mainly because theanimal?s behavior in the "on" state is a major constraint for multi-electrode chronic recordingstudies. To solve this and with the purpose to characterize the relationship of beta and highgamma (70?110Hz) cortical activity with parkinsonian state and the development of LIDs, wedesign a new setup based on a mouse spherical treadmill where animals could walk freelybut had the head restrained of movement. This system allowed us to record cortical LFP andspike signals by means of high density electrodes in hemiparkinsonian mice as well in animalswith LIDs, both in ?on? as in ?off? states. Movements were also tracked in order to correlatethem with brain activity. Preliminary results showing a characterization of the oscillatoryactivity in the beta range will be presented.