SPLENIC IRON PROTEINS RESPONSE IN MICE MODELS BY MEANS OF IRON EXCESS AND ERYTHROPOIESIS INDUCED BY ERYTHROPOIETIN SIGNALS

GIORGI GISELA; ALEJANDRA ELÍA; FERNANDEZ DELIAS MARIA FLORENCIA; ROQUE MARTA ELENA

Buenos Aires

Congreso; Reunion Conjunta de las Sociedades de Biociencia; 2017

SAIC

Introduction: Iron and erythropoiesis are closely related since the iron required for erythropoiesis is derived from the recycling of heme-iron by splenic macrophages. Hence, the spleen is susceptible to iron-related disorders for example the iron excess. Objective: To evaluate the effect of Erythropoietin in spleen when two states coexist, it was used an animal model of iron-excess and also an erythropoiesis induced by EPO. Materials and Methods: CF1 mice (25±5g; 3 months-old) were divided into groups (n=4/group): 1)Iron-adequate (control); 2)Iron-overload (iron saccharate; days 0,4,8,12 ip;1800mg/kg); 3)EPO (days17,18,19) ip; 20000UI/kg); 4)Iron-overload+EPO. Immunohistochemistry: anti-prohepcidin, L-ferritin and DMT1 (divalent metal transporter1). Perl´s staining. Erythropoietic response was assessed by hematological tests. The Protocol was approved by Committee on Experimental Animal Use and Care, UNS. Results: Prohepcidin expression was intense in Iron-overload respect to control, while it was weak in EPO and Iron-overload+EPO. DMT1 and L-ferritin expressions were increased in Iron-overload and decreased in EPO and Iron-overload+EPO compared to control. Hb (g/dl) and HCT (%) in Iron-overload+EPO (16,8±0,4;54±1) and EPO (17,1±0,7;56±2) showed a significant rise respect to Iron-overload (14,8±0,23;49±1) and control (15,5±0,5;45±1). Hemosiderin was intense in Iron-overload, while it was weak in EPO and Iron-overload+EPO. Plasma iron levels showed a significant increase in Iron-overload respect control, EPO and Iron-Overload+EPO states (p