PROTEINS ASSOCIATED TO UPTAKE OF IRON IN HUMAN NEUROBLASTOMA CELLS

ROQUE MARTA ELENA; FERNANDEZ DELIAS MARIA FLORENCIA; GIORGI GISELA; VITTORI DANIELA

Buenos Aires

Congreso; Reunion Conjunta de las Sociedades de Biociencia; 2017

SAIC

Introduction: Alterations in the neuronal iron uptake are frequently associated to brain diseases. Therefore, the aim was to study the effect of iron excess on divalent metal transporter1 (DMT1) and Zrt-Irt-like Protein14 (ZIP14) expressions in human neuroblastoma cells. Materials and Methods: To evaluate cellular viability a dose-response curve was assayed with low and high iron concentrations (ferric ammonium citrate-FAC) 30µM-80µM and 200µM-600µM, respectively at different times. The iron uptake was assessed in the culture medium in 30µMFAC/72hs and in 600 µM/24hs treatments. Pre-treated cells with NAC 2Mm/12hs plus FAC30µM/72hs for immunocytochemistry: DMT1, ZIP14, prohepcidin, ferritin. Viability was assessed by a nuclear red method. Iron levels: Wiener kit. Results: In treatments with low FAC concentrations (30µM, 60µM and 80µM) it was observed a decrease in cellular viability at 72hs (p