Bone and vascular biocompatibility of magnetic nanoparticles for targeted drug delivery

CEPEDA SABRINA; MASSHEIMER VIRGINIA; CAMPELO ADRIÁN; SANDOVAL MARISA; AGOTEGARAY MARIELA; LASALLE VERÓNICA

Buenos Aires

Congreso; 34 Reunión Anual AAOMM; 2017

AAOMM

Silica coated magnetic nanoparticles (MNPs) are potential devices for drug targeting. A magnetite core allows the MNPs to be directed to a specific site of the organism through the vascular system by the application of an external magnetic field for targeted delivery of drugs. An inert silica coating of the core confers the required biocompatibility for MNPs use in the biomedical field, and acts also as platform for the incorporation of biomolecules. Looking forward bone targeting of MNPs, the aim of the present work is to evaluate their vascular and bone biocompatibility. Primary cultures of rat aorta endothelial cells (EC) and osteoblasts (OB) were obtained by explant technique from Wistar rats. Cells were exposed to 24-48h treatment at doses of 0.1-100μg/ml of MNPs and specific cellular processes were evaluated.Cell viability was assayed in EC and OB (MTT assay). No effect of MNPs was observed below 10μg/ml. 100μg/ml of MNPs induced a 20% and 50% decrease in EC and OB viability respectively (p