OPPOSITE ACTIONS OF MEDROXYPROGES-TERONE ACETATE (MPA) ON BONE AND VASCULAR CELLS.

MASSHEIMER VIRGINIA; CUTINI PABLO

Mar del Plata

Congreso; LXI Reunión Científica anual de la Sociedad Argentina de investigación Clínica.; 2016

Sociedad Argentina de Investigación Clínica

A high bone turnover isassociated with increased cardiovas-cular mortality in aging. Hormone replacement therapy combined with progestins is proposed as an alternative choice in prevention of cardiovascular diseases. Vascular calcification (VCa) mainly involves vascular smooth muscle cells (VSMC) transdifferentia-tion to bone lineage. The aim of this work was to investigate the role of MPA on osteoblasts and osteoblasts derived from VSMC, as well as its impact on vascular calcification. The following murine cell cultures were used: a) VSMC; b) VSMC induced to osteoblastic transdifferentiation (VSMC-OB) in osteogenic me-dium (β-glycerophosphate 5 mM; CaCl2 and 4 mM); c) calvarial osteoblasts (OB). VSMC proliferation and migration, and inducible muscle nitric oxide (NO) synthesis are early events that conduct to VCa. We found that 96 h treatment with the progestin (10 nM) inhibited the VSMC growth (17.6% vs control, p