Opposite actions of medroxyprogesterone acetate (MPA) on bone and vascular cells

MASSHEIMER V.; CUTINI P.

Mar del Plata

Congreso; LXI Reunión Anual de la SAIC - LXIV Reunión Anual de la SAI - XLVIII Reunión Anual de la SAFE - VII Reunión Anual de la NANOMEDAR - V Congreso Nacional de la AACyTAL; 2016

A high bone turnover is associated with increased cardiovascular mortality in aging. Hormone replacement therapy combined with progestins is proposed as an alternative choice in prevention of cardiovascular diseases. Vascular calcification (VCa) mainly involves vascular smooth muscle cells (VSMC) transdifferentiation to bone lineage. The aim of this work was to investigate the role of MPA on osteoblasts and osteoblasts derived from VSMC, as well as its impact on vascular calcification. The following murine cell cultures were used: a) VSMC; b) VSMC induced to osteoblastic transdifferentiation (VSMC-OB) in osteogenic medium (β-glycerophosphate 5 mM; CaCl2 and 4 mM); c) calvarial osteoblasts (OB). VSMC proliferation and migration, and inducible muscle nitric oxide (NO) synthesis are early events that conduct to VCa. We found that 96 h treatment with the progestin (10 nM) inhibited the VSMC growth (17.6% vs control, p