Bone-vascular-adipose tissue interplay: modulation by estrogens

CEPEDA S.; VALLE M.; CUTINI P.; MASSHEIMER V.; SANDOVAL M.; CAMPELO A.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2022; 2022

Nitric oxide (NO) promotes bone cells proliferation, differentiation and survival. An adequate vascularization that provides cell progenitors and hormones is required for skeletal homeostasis. The menopausal hypoestrogenism is tightly associated with bone and cardiovascular diseases, and also with changes in adipose tissue (AT) distribution. In this work we studied the role of the estrogens estradiol (E2) and estrone (E1), and phytoestrogen genistein (Gen) on bone-vascular or bone-AT interactions. Two experimental designs were employed: 1) conditioned medium obtained from endothelial cells (EC) exposed to Gen (CM-EC), or conditioned medium obtained from osteoblasts (OB) exposed to Gen (CM-OB); 2) co-cultures OB-AT. A bidirectional regulation between OB and EC was revealed. CM-EC added to OB monolayers stimulated bone cells proliferation (150% a/C, p