Structural changes in endocrine pancreas of male Wistar rats due to chronic cola drink consumption.

MORIONDO, MARISA MABEL

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2021

1932-6203

AbstractAim: The objective of this work was to analyze the structural changes of the pancreaticislets in rats, after 6 month consuming regular and light cola for 6 months. Also, we haveanalyzed the possible role of PDX-1 in that process. Finally, with the available knowledge,we propose a general working hypothesis that explains the succession of phenomenaobserved. Previously, we reported evidence showing that chronic cola consumption in ratsimpairs pancreatic metabolism of insulin and glucagon and produces some alterations typi-cally observed in the metabolic syndrome, with an increase in oxidative stress. Of note It isworth mentioning that no apoptosis nor proliferation of islet cells could be demonstrated. Inthe present study, 36 male Wistar rats were divided into three groups to and given freeaccess to freely drink regular cola (C), light cola (L), or water (W, control). We assessed theimpact of the three different beverages in on glucose tolerance, lipid levels, creatinine levelsand immunohistochemical changes addressed for the expression of insulin, glucagon, PDX-1 and NGN3 in islet cells, to evaluate the possible participation of PDX-1 in the changesobserved in α and β cells after 6 months of treatment. Moreover, we assessed by stereologi-cal methods, the mean volume of islets (Vi) and three important variables: the fractional β-cell area, the cross-sectional area of alpha (A α-cell) and beta cells (A β-cell), and the num-ber of β and α cell per body weight. Data were analyzed by two-way ANOVA followed byBonferroni?s multiple t-test or by Kruskal-Wallis test, then followed by Dunn?s test (depend-ing on distribution). Statistical significance was set at p