DIFFERENTIAL EFFECT BY SEX OF A HIGH FAT DIET IN C57/BL6J MICE BEHAVIOUR AND GLUCIDIC METABOLISM. INFLUENCE OF CHRONIC MILD STRESS.

PROCHNIK ANDRÉS; GONZÁLEZ MURANO MARÍA ROSA; MIRIAM WALD; GENARO ANA MARÍA

Mar del Plata

Congreso; XLVIII REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGIA EXPERIMENTAL-SAFE; 2016

SAIC SAI SAFE AACYTAL

DIFFERENTIAL EFFECT BY SEX OF A HIGH FAT DIET IN C57/BL6J MICE BEHAVIOUR AND GLUCIDIC METABOLISM. INFLUENCE OF CHRONIC MILD STRESS.Prochnik A. 1 , González Murano M.R. 1 , Wald M.R. 1 ,Genaro A.M. 1,2(1) BIOMED, UCA ?CONICET(2) Dto de Farmacología, Facultad de Medicina, UBA                  In the past decades overweight and obesity have turned into one of the most common non-contagious diseases, currently spreading worldwide. In recent clinical studies obesity has been associated with an impairment in cognitive function and even pointed at being a risk factor in the development of dementias, such as Alzheimer?s disease. In the present study we analyze if a high fat diet can lead to behaviour alterations and wether these are associated with metabolic changes. Furthermore, we investigated if stress aggravates these alterations. Male and female C57/BL6J mice were fed with a high fat diet (HFD; 35% w/w) or a standard diet (SD, 5% w/w) during 6 months. 2 months after starting the diet, mice under HFD were subdivided: A group received chronical mild stress (CMS) and the other served as control. By the end of the treatment we measured body weight , performed behaviour tests relevant to anxiety, context and spacial memory and run a glucose tolerance test. Results are: Mice under HFD had a higher body weight, which reverted with CMS (Male mice:N=9; p<0,05. Female mice:N=9;p<0,01). Under HFD, males had higher glucose intolerance, which worsen with CMS (N=9. p<0,05). Also, HFD males performed worse in spatial object recognition (SOR) (N=4;p<0,05) while CMS caused higher anxious-like behaviour (N=9.p<0,01). We also found a negative correlation between glucose intolerance and SOR results (Correlation:-0,6257;p<0,05) and glucose intolerance and anxious-like behaviuor (Correlation:-0,4798;p<0,05). On the other hand, the treatments seem to have no effect on females neither in their metabolism nor their behaviour. Evidence shows that, in this model, gender doesn?t prevent the body weight increase with a HFD, however it is an important factor in the development of disorders associated with overweight, both metabolic and behavioural. Moreover, in males stress has a paradoxical effect preventing weight gain but disregulating the glucidic metabolism.