INVOLVEMENT OF TOLL LIKE RECEPTOR 4 (TLR4) IN THE EFFECT OF HIGH-FAT DIET AND/OR CHRONIC STRESS

QUIROGA SOFÍA; MARCONE MARÍA PAULA; GONZÁLEZ MURANO, MARÍA ROSA; VIDAL MARÍA AGUSTINA; GENARO ANA MARÍA

Mar del Plata

Congreso; Reunión Conjunta SAIC SAI SAFIS 2022; 2022

SAIC SAI SAFIS

INVOLVEMENT OF TOLL LIKE RECEPTOR 4 (TLR4) IN THE EFFECT OF HIGH-FAT DIET AND/ORCHRONIC STRESSSofía Quiroga, María Paula Marcone, María Rosa González Murano, María Agustina Vidal, AnaMaría Genaro*, Adriana Laura Burgueño*.Instituto de Investigaciones Biomédicas (BIOMED)/UCA CONICET* both authors contributed equally to the workHigh fat diet (HFD) or chronic stress (CS) lead to low-grade inflammation, promotingneuroinflammation and memory impairment in rodents. To analyze the role of theinflammasome NLRP3 in these alterations we compared the effect of HFD and CS in C57Bl/6J(WT) and TLR4 KO mice. We previously found that HFD in WT increases body weight and basalglycemia levels. These results were not observed in KO mice. The aim of this study was to analyzethe effect of HFD and/or CS on cognition and gene expression in hippocampus from WT and KOmice.At 4 weeks of age, males received standard diet (SD) or HFD. After 12 weeks, they were exposed(or not) to CS for 8 weeks, resulting in 4 groups for each strain: 1) SD, 2) SD+CS, 3) HFD, and 4)HFD+CS. To assess cognitive performance, we conducted the Object location test (OLT).Hippocampal mRNA expression levels of PYCARD, NLRP3 (components of NLRP3 inflammasome)and NGF, BDNF and SIRT1 (as markers for cognitive deficits) were assess by qPCR.We found that OLT was altered in WT HFD or CS (p