Increased recruitment of granulocytes in the ovaries of dairy cows with cystic ovarian disease (COD).

ETCHEVERS L; CAINELLI, S.; ORTEGA, H.H.; STASSI A.F.; VILLALBA L; REY, F.; ACOSTA V; SALVETTI, N.R.

Mar del Plata

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

Sociedad Argentina de Investigación Clínica

COD is among the most relevant cause of subfertility in dairy cattle and is the result of ovulation failure. Ovulation has been characterized as an inflammatory process in which granulocytes are involved. Therefore, the aim of this study was to evaluate the distribution of granulocytes (CH138+) in ovaries of animals with spontaneous COD and control (C) animals. Ovariectomy was performed in animals with COD to obtain ovaries (n = 5), also control cows were ovariectomized in proestrus (n = 5). CH138+ cells were evaluated through immunohistochemistry in total ovary (TO), in ovarian cortex (OC), medulla (OM), theca interna (TI) and externa (TE) of cysts and dominant follicles. In addition, CH138+ cells were evaluated by immunocytochemistry performed on samples of follicular fluid from (FF) cysts and dominant follicles. The specificity of the antibody was corroborated by western blot (WB). The data was analyzed using ANOVA with Duncan's post-test and T-student test. WB analysis detected a strong positive band of 17 KDa for CH138. Comparison between groups showed that, the number of CH138+ cells was higher in TO, OM and TE of COD group than in C group (p