Host Epac1 is required for cAMP-mediated invasion by Trypanosoma cruzi

MUSIKANT, D.; FERRI, G.; DURANTE IGNACIO; CARLOS ANDRES BUSCAGLIA; ALTSCHULER, D.; EDREIRA, M.

MOLECULAR AND BIOCHEMICAL PARASITOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017 vol. 211 p. 67 - 70

0166-6851

Mechanistic details of the modulation by cAMP of Trypanosoma cruzi host cell invasion remain ill-defined.Here we report that activation of host?s Epac1 stimulated invasion, whereas specific pharmacological inhi-bition or maneuvers that alter Epac1 subcellular localization significantly reduced invasion. Furthermore,while specific activation of host cell PKA showed no effect, its inhibition resulted in an increased invasion,revealing a crosstalk between the PKA and Epac signaling pathways during the process of invasion. There-fore, our data suggests that subcellular localization of Epac might be playing an important role duringinvasion and that specific activation of the host cell cAMP/Epac1 pathway is required for cAMP-mediated invasion.