Electrochemical Magnetic Microbeads-based Biosensor for Point-of-Care Serodiagnosis of Infectious Diseases

CORTINA, M.E.; MELLI LUCIANO; REY SERANTES DIEGO; YBARRA, G.; LONGINOTTI, M.G.; LLORET, P.; RESTUCCIA, S.; ROBERTI, M.; MASS, M.; TROPEA, S.; ALTCHEH JAIME; CARLOS ANDRES BUSCAGLIA; FRAIGI L; MOINA CARLOS; UGALDE JUAN; CIOCCHINI ANDRES; COMERCI DIEGO

BIOSENSORS & BIOELECTRONICS

ELSEVIER ADVANCED TECHNOLOGY

Lugar: Amsterdam; Año: 2016 vol. 80 p. 24 - 33

0956-5663

Access toappropriatediagnostictoolsisanessentialcomponentintheevaluationandimprovementof global health.Additionally,timelydetectionofinfectiousagentsiscriticalinearlydiagnosisandtreat- ment ofinfectiousdiseases.Conventionalpathogendetectionmethodssuchasculturing,enzymelinked immunosorbent assay(ELISA)orpolymerasechainreaction(PCR)requirelongassaytimes,andcomplex and expensiveinstrumentsmakingthemnotadaptabletopoint-of-care(PoC)needsatresource-con- strained placesandprimarycaresettings.Therefore,thereisanunmetneedtodevelopportable,simple, rapid, andaccuratemethodsforPoCdetectionofinfections.Here,wepresentthedevelopmentand validationofaportable,robustandinexpensiveelectrochemicalmagneticmicrobeads-basedbiosensor (EMBIA) platformforPoCserodiagnosisofinfectiousdiseasescausedbydifferenttypesofmicroorgan- isms (parasiticprotozoa,bacteriaandviruses).WedemonstratethepotentialuseoftheEMBIAplatform for in situ diagnosis ofhuman(Chagasdiseaseandhumanbrucellosis)andanimal(bovinebrucellosisand foot-and-mouth disease)infectionsclearlydifferentiatinginfectedfromnon-infectedindividualsoran- imals. ForChagasdisease,amoreextensivevalidationofthetestwasperformedshowingthattheEMBIA platform displayedanexcellentdiagnosticperformancealmostindistinguishable,intermsofspecificity and sensitivity,froma fluorescent immunomagneticassayandtheconventionalELISAusingthesame combination ofantigens.Thisplatformtechnologycouldpotentiallybeapplicabletodiagnoseother infectious andnon-infectiousdiseasesaswellasdetectionand/orquantification ofbiomarkersatthe POC andprimarycaresettings.