Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth

MARIAVITTORIA PIZZINGA; CHRISTIAN BATES; JENNIFER LUI ; GABRIELLA FORTE; FABIAN MORALES-POLANCO; EMMA LINNEY; BARBORA KNOTKOVA; BEVERLEY WILSON; CLARA A. SOLARI; LUKE E. BERCHOWITZ; PAULA PORTELA; MARK ASHE

JOURNAL OF CELL BIOLOGY

ROCKEFELLER UNIV PRESS

Lugar: New York; Año: 2019

0021-9525

mRNA localization serves key functions in localized protein production, making it critical that the translation machinery itselfis present at these locations. Here we show that translation factor mRNAs are localized to distinct granules within yeast cells.In contrast to many messenger RNP granules, such as processing bodies and stress granules, which contain translationallyrepressed mRNAs, these granules harbor translated mRNAs under active growth conditions. The granules require Pab1p fortheir integrity and are inherited by developing daughter cells in a She2p/She3p-dependent manner. These results point to amodel where roughly half the mRNA for certain translation factors is specifically directed in granules or translation factoriestoward the tip of the developing daughter cell, where protein synthesis is most heavily required, which has particularimplications for filamentous forms of growth. Such a feedforward mechanism would ensure adequate provision of thetranslation machinery where it is to be needed most over the coming growth cycle.