INVESTIGADORES
PORTELA Paula
artículos
Título:
Activation state of protein kinase A as measured in permeabilised Saccharomyces cerevisiae cells correlates with PKA-controlled phenotypes in vivo
Autor/es:
PORTELA P; VAN DIJCK P; THEVELEIN J; MORENO S
Revista:
FEMS YEAST RESEARCH
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2003 vol. 3 p. 119 - 126
ISSN:
1567-1356
Resumen:
Protein kinase A (PKA) activity was measured in situ in permeabilised Saccharomyces cerevisiae cells in the absence and the presence ofcAMP. Four strains genetically predicted to have differential PKA-dependent phenotypes were used: a wild-type strain and a straincontaining a bcy1-14 mutation (with almost constitutively active PKA), and the same strains with overexpression of the wild-type ormutant BCY1 gene, respectively. Cells were grown on galactose or glucose. The measured phenotypic characteristics were: trehalose andglycogen levels and the activity of a reporter gene under control of the NTH1 promoter. The ?endogenous? PKA activity (measured in situin the absence of cAMP) showed the best correlation with the PKA-dependent phenotypes determined in vivo. We propose that thisparameter offers a good estimate for the degree of activation of PKA in vivo.
