INVESTIGADORES
DEL PAPA Maria Florencia
artículos
Título:
Development of new positive-selection RIVET tools: detection of induced promoters by the excision-based transcriptional activation of an aacCI (GmR)-gfp fusion
Autor/es:
LOZANO, M. J.; SALAS, M.E.; GIUSTI ,M.A.; DRAGHI, W. O.; TORRES TEJERIZO, G.A.; MARTINI, M.C.; DEL PAPA, M.F.; PISTORIO, M.; LAGARES, A.
Revista:
JOURNAL OF BIOTECHNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011 vol. 155 p. 147 - 155
ISSN:
0168-1656
Resumen:
RIVET (Recombination Based in-vivo Expression Technology) is a powerful genetic tool originally conceived for the identification of genes induced in complex biological niches where conventional transcriptomics is difficult to use. With a broader application, genetic recombination-based technologies have also been used, in combination with regulatory proteins and specific transcriptional regulators, for the development of highly-sensitive biosensor systems. RIVET systems generally comprise two modules: a promoter-trap cassette generating genomic transcriptional fusions to the tnpR gene encoding the Tn-gd TnpR resolvase, and a reporter cassette carrying res-flanked selection markers that are excised upon expression of tnpR to produce an irreversible, inheritable phenotypic change.
