INVESTIGADORES
DEL PAPA Maria Florencia
artículos
Título:
Novel tnpR-based transposable promoter traps suitable for RIVET studies in different Gram-negative bacteria.
Autor/es:
LOZANO M.J.; SALAS, M.E.; GIUSTI ,M.A.; MARTINI M.C.; LOPEZ; SALTO; DEL PAPA M. F.; PISTORIO M; LAGARES A
Revista:
JOURNAL OF MICROBIOLOGICAL METHODS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 93 p. 9 - 11
ISSN:
0167-7012
Resumen:
The preparation of plasmid-borne RIVET libraries can be troublesomewhen high genomic coverages are needed.We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by Transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.
