Assessment of flickering chromatic pupillometry in patients with risk of glaucoma

MARÍA CONSTANZA TRIPOLONE; LUIS ISSOLIO; CARLOS AGÜERO; ALEJANDRO LÁVAQUE; PABLO PÉREZ; PABLO BARRIONUEVO

Denver

Congreso; ARVO 2022; 2022

Association for Research in Vision and Ophthalmology

Purpose : Chromatic pupillometry is increasingly used for assessing human retinal function through measurements of pupil responses to different colored light stimuli. The discovery of intrinsically photosensitive retinal ganglion cells (ipRGCs) adds a new dimension to the detection and monitoring of retinal and optic nerve disorders, including open-angle glaucoma (OAG) through pupillometry. However, the detection of pre-perimetric glaucomatous deficits still remains a challenge. We hypothesize that early damage in OAG risk could be reflected in affected flickering pupil responses.Methods : All participants underwent ophthalmological assessments and were classified in healthy controls (HG) and OAG risk patients (GR). The GR group was characterized with high intraocular pressure and/or a family history of OAG with no pre-existing OAG; assessed by OCT and ERG. Pupillary recordings were obtained using a lab-made photostimulator-pupillometer system with monochromatic stimuli of blue (468 nm), green (516 nm), and red (632 nm). Flickering sinusoidal stimuli of 1Hz frequency and 10s duration were presented in an integrating sphere-like dome with a Lambertian inner surface at two modulation levels: 100% and 70%. Each participant was monocularly tested and was light-adapted to the mean chromaticity during 60 s., previous to the stimulus onset. In the frequency domain, amplitude (mm) and phase (degrees) parameters were assessed. In the temporal domain (% initial diameter), initial constriction, and post pulse plateau parameters were evaluated.Results : The amplitude was significantly reduced (p