Bacterioruberin extracts from a genetically modified hyperpigmented Haloferax volcanii strain: Antioxidant activity and bioactive properties on sperm cells

LUCIA ZALAZAR; PABLO PAGOLA; MARÍA VICTORIA MIRÓ; MARÍA SANDRA CHURIO; MICAELA CERLETTI; CELESTE MARTÍNEZ; MARÍA INIESTA-CUERDA; ANA JOSEFA SOLER VALLS; ANDREINA CESARI; ROSANA DE CASTRO

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2019

1364-5072

Aims: To examine the antioxidant activity of Bacterioruberin (Bctr)-rich extracts isolated from a hyperpigmented-genetically modified Haloferax volcanii strain (HVLON3) and to investigate the effect on cold-sensitive ram sperm cells.Methods and Results: The strain HVLON3 produces higher Bctr amounts than most haloarchaea (220 ± 13 mg/g DW). HVLON3-Bctr extract has higher antioxidant activity than β-carotene (3-fold) as evaluated using 2,2 diphenyl-1-picrylhydrazyl combined with Electron Paramagnetic Resonance analysis (EC50 4.5 x 10-5 mol l-1 vs. 13.9 x 10-5 mol l-1, respectively). Different concentrations of HVLON3-Bctr extracts were assayed on ram sperm after freezing/thawing and physiologically relevant parameters were examined. Extracts containing 7 and 20 µmol l-1 Bctr significantly improved cell viability (p