The Mouse Sperm Rosette (MSR): assembling during epididymal transit, in vitro disassemble and oligosaccharides participation in the linkage material.
MONCLUS MA; CESARI A; CABRILLANA ME; BOARELLI PV; VINCENTI AE; BURGOS MH; FORNÉS MW
The Anatomical Record
Año: 2007 vol. 290 p. 814 - 824
In many mammals sperm associations had been observed, but not in the mouse. In this work the Mouse Sperm Rosettes are morphologically described inside the epididymis and during its dissolution in a culture media. It was also characterized the saccharides present in the linking material. Sperm association and others epididymal actions are supported by sperm during the epididymal transit and verified at the caudal region, suggesting a relation between epididymal transit and sperm maturation. In drops of epididymal content obtained from distal (cauda), but not from proximal regions (caput and corpus), dissolved in culture media Rosettes appear constituid by 10 to 15 motile sperm joined by their heads. After 3 minutes sperm progressively detach disassembling the Rosette. This structures are studied by several techniques including optic, electronic (SEM and TEM) and video microscopy. At ultra structural level a dense network of electron dense material was observed between sperm heads, joining them. Based on previous works in rat, several lectins were used to characterize the type of saccharides present in this linking material. In order to avoid the contact between sperm and epididymal fluid from distal region - that probably exerts an influence on sperm association- a ligature was placed between caput and corpus. This epididymal content isolated from caput did not display any Rosettes after 28 days.