In vitro effect of glyphosate on aflatoxin B1 productio by Aspergillus section Flavi.

BENITO N.; CARRANZA C.S.; ALUFFI M.E.; MAGNOLI C.E,; BARBERIS C.L.

Minas Gerais, Bello Horizonte

Congreso; XVI Encuentro Nacional de Micotoxinas; 2018

Glyphosate is the most used herbicide on regional agricultural practices in Argentina. There is limited information about the impact of this herbicide on mycotoxins production of fungal strains that commonly colonize agricultural crops. The objective of this study was evaluate the in vitro effect of different concentrations of glyphosate on aflatoxin B1 (AFB1) production by Aspergillus section Flavi strains under different water activity (aW) and incubation periods. Yeast Extract, Sucrose medium (YES) was adjusted to 0,99; 0,96 and 0,92 of aW, and supplied with 30 and 300 mM of glyphosate (commercial formulation) and incubated at 25 °C for 21 days of incubation. AFB1 was detected by HPLC at 7, 14 and 21 of incubation days. The optimal conditions for the accumulation of AFB1 in control treatments were 0,92 aW at 21 days of incubation, for Aspergillus flavus AFM16. The highest levels of AFB1 production was recorder with 30 mM of herbicide for NRRL 2999 at 21 days of incubation. All strains, except AFM 16, showed the maximum levels production with 30 mM of glyphosate at 21 days. These results are important to understand the aflatoxigenic consequences that the extensive use of the herbicide glyphosate may cause on maize crops