Determination of residues of afb1 in the liver of broiler chicken by HPLC chromatography-mass / mass.

MAGNOLI A.P.,; MONGE M.P.,; CAVAGLIERI L.,; DALCERO A.M.,; MAGNOLI C.E.,; CHIACCHIERA S.M.

Merida, Yucatán, México

Congreso; VI Latin American Congress of Mycotoxicology and II International Symposium on Fungal and Algal Toxins in Industry; 2010

Aflatoxin B1 (AFB1), a mycotoxin produced by Aspergillus flavus and Aspergillus parasiticus, is known to be hepatotoxic and hepatocarcinogenic in several animal species, especially young poultry (Ortatatli and Oguz, 2001). In vivo studies have shown that the liver is the predominant target organ for AFB1 (Miller and Wilson, 1994). Aflatoxin contaminated feed are a severe threat to both livestock productivity and human health and thus, brings huge worldwide economic losses each year ( (Diaz, 2005). A large number of the observations have shown that hepatic metabolism plays a prominent role in determining the biological action of AFB1. Conversion of AFB1 to aflatoxin M1, aflatoxin P1, aflatoxin Q1, aflatoxin B2a and aflatoxicol are regarded to be detoxification steps, because the toxicity or carcinogenicity of these metabolites are lower than those of aflatoxin B1 (Larsson and Tjalve, 1992). However, AFB1 in liver may remain unchanged even when exposure levels are relatively low. The present work deals with the evaluation of AFB1 in livers of broilers fed diets contaminated with 50 ng of the toxin per g of feed