Antiochratoxigenic effect of propylparaben on Aspergillus section Nigri strains on peanut grains.

BARBERIS C.; MONGE M.P.,; LANDA F.,; DALCERO A.M.,; MAGNOLI C.

Tulln, Austria

Congreso; ISM Conference 2009. Worldwide Mycotoxins Reduction in Food and Feed Chains; 2009

The aim of the present study was to evaluate the effect of one of the best food grade antioxidant, propylparaben on i) the lag phase to growth, ii) growth rates and iii) ochratoxin A (OTA) production by Aspergillus niger aggregate strains under different environmental conditions on peanut grains. Two A. niger aggregate strains (RCP42 and RCP191) were evaluated. Irradiated peanut grains (7 kGy) were dehydrated to 0.980, 0.950 and 0.930 aW. The antioxidant propyl 4- hydroxybenzoate (PP) were added to the irradiated grains to obtain the required concentrations (1, 5, 10 and 20 mmol g-1). Peanut grains were inoculated centrally with 5 µl of spore suspensions (1 x 106 spores ml-1) and incubated at 18 and 25°C for 30 days. Growth parameters and OTA concentration were determined. Ochratoxin A extraction was carried out using a clean up column (MycoSep ®229 Ochra column, MFC, Romer Labs®, Inc., MO., USA) and detected by HPLC. Propyl paraben completely inhibit growth rate in the highest concentration.