Aislamiento e identificación de las especies fúngicas de suelo que integran el consorcio con capacidad para degradar glifosato en condiciones in vitro.

ALUFFI M.E.; CARRANZA C.S.; BENITO N.; CHIACCHIERA S.,; BARBERIS C.L.; MAGNOLI C.E,

Luján, Buenos Aires

Congreso; V Congreso Nacional de Ecología y Biología de Suelo.; 2017

The use of agricultural pesticides for insect, pest and disease control has increased during the past years in Argentina. The orgahophosphonates as glyphosate are biogenic and xenobiotic compounds characterized by the presence of a stable carbon to phosphorous (C-P) bond. The (C-P) bond imparts upon these molecules a relative resistance to degradation and concern has been expressed over their environmental impact and possible ecotoxicity. Therefore, it is required to develop bioremediation methods capable of degrading GP. The aim of this study was to evaluate the effect of several doses of GP on the growth parameters of different fungal strains isolated from agricultural soils. Twenty fungal strains were isolated from soils thought an enrichment method. Growth parameters were evaluated at different GP concentrations (0, 5, 20, 50 and 100 mM) at 0.98 of water activity on Czapek-Dox medium. In general, it was observed that the lag phase of all strains tested increased when GP concentration increased. The strains with the highest growth rate on treatment with 100 mM of GP were: C166 (Mucor spp), C2 (Aspergillus spp), C140 and C311 (Trichoderma spp). Growth rate and lag phase were : 4.13, 4.15, 5.01 and 6.39 mm/day and 9.65, 20.06, 46.2 and 32.69, respectively. These results show that these strains could integrate a microbial consortium capable to tolerate and degrade glyphosate on soil.