Brucella abortus Omp19 recombinant protein subcutaneously co-delivered with an antigen enhances antigen-specific T helper 1 memory responses and induces protection against parasite challenge

CORIA LM; IBANEZ AE; PASQUEVICH K; GONZALEZ COBIELLO PL; FRANK FM; GIAMBARTOLOMEI G; CASSATARO J

VACCINE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2016 vol. 20 p. 430 - 437

0264-410X

The discovery of effective adjuvants for many vaccines especially those with limited commercial appeal,such as vaccines to poverty-related diseases, is required. In this work, we demonstrated that subcutaneousco-administration of mice with the outer membrane protein U-Omp19 from Brucella spp. plus OVA as antigen (Ag) increases Ag-specific T cell proliferation and T helper (Th) 1 immune responses in vitro and in vivo. U-Omp19 treated dendritic cells promote IFN- production by specific CD4+ T cells and increases T cell proliferation. U-Omp19 co-administration induces the production of Ag specific effector memory T cell populations (CD4+ CD44high CD62Llow T cells). Finally, subcutaneous co-administration of U-Omp19 with Trypanosoma cruzi Ags confers protection against virulent parasite challenge, reducing parasitemiaand weight loss while increasing mice survival. These results indicate that the bacterial protein U-Omp19 when delivered subcutaneously could be a suitable component of vaccine formulations against infectious diseases requiring Th1 immune responses